Investigating the expression pattern of the OsAPx1 gene promoter in rice
نویسندگان
چکیده
Background Ascorbate peroxidase (APx) is a key enzyme of the antioxidant metabolism, catalyzing the decomposition of hydrogen peroxide (H2O2) in water, using ascorbate as an electron donor. The H2O2 is a reactive oxygen species (ROS) produced constantly by aerobic metabolism. Under biotic and abiotic stress the level of H2O2 increases and, in large quantities, can cause cellular damage. In rice, there are eight APx genes that encode products target to different subcellular compartments: cytosol, peroxisoma, mitochondria and chloroplast. OsAPx1 gene encodes a cytosolic isoform of APx. The study of promoters is an important tool that allows to analyze the overall expression pattern of genes in plants.
منابع مشابه
Expression Pattern of the Synthetic Pathogen-Inducible Promoter (SynP-FF) in the Transgenic Canola in Response to Sclerotinia sclerotiorum
Sclerotinia sclerotiorum is a phytopathogenic fungus which causes serious yield losses in canola. A pathogen inducible-promoter can facilitate the production of Sclerotinia-resistant transgenic canola plants. Inthis study, the “gain of function approach” was adopted for the construction of a pathogen-inducible promoter.The synthetic promoter technique was used, which involved the in...
متن کاملEvaluation of MYB93 and MAD8 Genes in Transgenic and Non-Transgenic Rice
Increasing drought tolerance, especially in rice, which is one of the most important crops in Asia, is necessary. Transcription factors are specific sequence DNA-binding proteins that are capable of activating or suppressing transcription. These proteins regulate gene expression levels by binding to cis regulatory elements in the promoter of target genes to control various biological processes ...
متن کاملGene Expression and Promoter Methylation Status of VHL, Runx-3, E-cadherin, P15 and P16 Genes During EPO-Mediated Erythroid Differentiation of CD34+ Hematopoietic Stem Cells
Background: VHL (von Hippel-Lindau), Runx-3 (Runt-related transcription factor 3), E-cadherin (Epithelial cadherin), P15 (INK4a, cyclin dependent kinase inhibitor), and P16 (INK4b) genes are essential in hematopoiesis. The aim of this study was to explore the correlation between gene expression and promoter methylation in CD34+ stem cells before and after differentiation to erythroid lineage. M...
متن کاملIndependence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA
MdMYB10 gene expression results in accumulation of anthocyanin in many tissues including flesh of applefruit. The MdMYB1 and MdMYBA genes are close homologues to MdMYB10 gene and both are responsiblefor red color phenotype in apple fruit skin. In the current study, an apple genome sequence draft analysisindicated that these three genes are located in a unique contig. Further a...
متن کاملGenetic polymorphism and expression analysis of cMBL gene in Iranian native and commercial chickens
The aims of this study were to compare the promoter sequence of the mannose-binding lectin (cMBL) gene in Iranian native and commercial chicken strains; as well as to compare the cMBL gene expression in crossbred and inbred chickens. In total 79 native (Western Azerbaijan native fowls, WANF) and 49 commercial (Arian Commercial Strain, ACS) birds were reared as parents under same management prac...
متن کامل